学术讲座【Bioartificial Kidney Development】

时间：2015年5月29日（周五）15:00-16:00

地点：仓山校区光电学院科研楼四层学术报告厅

主讲：Institute of Bioengineering and Nanotechnology (IBN), A*Star    Dr. Ming Ni

主办：光电与信息工程学院

专家简介：Ming Ni was born in Beijing, P. R. China. He attended University of Science and Technologies of China (USTC) to receive his BS degree in materials science and engineering. After that, he performed doctoral research at the University of Washington (Seattle) under the direction of Dr. Buddy Ratner (a member of US National Academy of Engineering), and received his PhD in Chemical Engineering in 2004. From 2005 to 2007, he was a postdoctoral fellow at the University of Pennsylvania working with Dr. Paul Ducheyne. In 2007, Dr. Ming Ni moved to Singapore to start his scientific career at the Institute of Bioengineering and Nanotechnology (IBN), A*Star. He is currently a research scientist at IBN. Dr. Ni’s research involves the use of biomaterials science in the development of tissue engineering scaffolds, drug delivery vehicles, stem cell culture substrates and imaging probes. Most recently, he works on peptide-based biomaterials and bioartificial kidney development.

报告摘要：Bioartificial kidneys (BAK) contain renal proximal tubule cells grown on porous membranes, which are typically coated with an extracellular matrix (ECM). Human primary renal proximal tubule cells (HPTCs) are selected as they are the most relevant cell type for clinical applications. Commercially available hollow fiber membrane materials, such as polyethersulfone/polyvinylpyrrolidone (PES/PVP), polysulfone/polyvinylpyrrolidone (PSF/PVP) and regenerated cellulose (RC), were chosen as porous membranes because they are used for hemodialysis. However, we found that these membrane materials do not support HPTCs’ growth and survival. To solve this problem, we have applied a variety of surface treatments and extracellular matrix coatings to PES/PVP and PSF/PVP membranes. Our results showed that double coating with 3,4-dihydroxy-L-phenylalanine (DOPA) and an ECM markedly improves HPTC performance on PSF/PVP. We have also synthesized alternative membrane materials made of polysulfone and FullcureTM. We found that these membrane materials sustain proper HPTC performance without the need of surface treatments or coating. An overview of the work will be presented.